Van Niekerk, who broke Johnson’s 400m world record of 43.18 in Rio with a 43.03, ran 30.81 seconds in Ostrava. Video is here.
Johnson’s previous world best in the rarely contested event was 30.85, clocked at altitude in Pretoria in 2000, his final season.
Van Niekerk previously ran 31.03 over 300m in Kingston, Jamaica, last year. At the time, only Johnson and Usain Bolt (30.97 in 2010, also in Ostrava) had run faster in the non-Olympic event.
Johnson, also the former 200m world-record holder (lowered by Bolt in 2008 and 2009), is left with one world record still standing from the 1993 World Championships 4x400m relay.
Van Niekerk is preparing for the world championships in August in London, where he is expected to contest the 200m and 400m.
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